The steady-state rate of ATP synthesis in the perfused rat heart measured by 31P NMR saturation transfer |
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Authors: | PM Matthews JL Bland DG Gadian GK Radda |
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Institution: | Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, U.K. |
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Abstract: | The steady-state rate of ATP synthesis in the isolated, Langendorff-perfused rat heart was determined using a 31P NMR saturation transfer method. At 37°C and a perfusion pressure of 70 cm H2O the value is 2.8 ± 0.3 (n=5 ± S.E.M.) μmol.s?1. (g. dry wt.)?1. The activity of creatine phosphokinase measured in the same experiments was 14.6 ± 1.0 μ mol.s?1 .(g. dry wt.)?1. From the rate of ATP synthesis and the separately measured oxygen consumption we calculated an apparent mitochondrial ADP:O ratio of 3.5 ± 0.8 in the intact tissue. |
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