Studies on the enzymic degradation of luteinizing hormone releasing hormone by rat pituitary plasma membranes |
| |
Authors: | S Elkabes M Fridkin Y Koch |
| |
Institution: | 1. Department of Hormone Research, The Weizmann Institute of Science, Rehovot, Israel.;2. Department of Organic Chemistry, The Weizmann Institute of Science, Rehovot, Israel. |
| |
Abstract: | Degradation of luteinizing hormone releasing hormone (LH-RH) by purified plasma membranes from rat pituitaries was investigated. Synthetic LH-RH (0.5 mg/ml) was incubated (20 min, 37°C) with pituitary plasma membranes (750 μg protein/ml). The reaction was stopped by centrifugation at 4°C. The degradation products were isolated by high pressure liquid chromatography using a reversed-phase column. Amino acid analysis of the degradation products indicated that the N-terminal tripeptide (pGlu-His-Trp) and the N-terminal hexapeptide (pGlu-His-Trp-Ser-Tyr-Gly) sequence of LH-RH are the main degradation products. These results suggest that the main cleavage sites of LH-RH by the pituitary plasma membrane-bound enzymes are the Gly6-Leu7 and the Trp3-Ser4 bonds of the neurohormone. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|