Protection of steroid hormone receptors by protease inhibitors |
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| Authors: | Tadahiko Hazato Akira Murayama |
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| Affiliation: | Department of Immunology The Tokyo Metropolitan Institute of Medical Science Honkomagome, Bunkyoku, Tokyo 113, Japan |
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| Abstract: | Steroid hormone receptors are proteolyzed by different types of enzymes present in target tissues. Effective protease inhibitors protecting steroid hormone receptors in various target tissues were investigated. Progesterone receptor (PR) in hen oviduct and estrogen receptor (ER) in cow uterus were specifically protected by relatively low concentrations (0.5 mM) of leupeptin or antipain (inhibitors of serine and thiol proteases). It was indicated that two different types of enzymes which modify native glucocorticoid receptor (GR) are present in rat liver. One was inhibited by 1 mM leupeptin or 1 mM antipain, while the other was inhibited by 1 mM phosphoramidon (inhibitor of thermolysin like proteases) or 10 mM sodium molybdate. Native PR, ER, and GR were shown to have similar Stokes radii (44 Å). |
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| Keywords: | ER estrogen receptor PR progesterone receptor GR glucocorticoid receptor TEM buffer 10 mM Tris-HCl, 1.5 mM EDTA, 1.5 mM 2-mercaptoethanol, pH 8.0 at 2°C TESH buffer 10 mM Tris-HCl, 1.5 mM EDTA, 10 mM thioglycerol, pH 8.0 at 2°C |
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