Isolation of hexose-6-phosphate dehydrogenase from rat liver microsomal fraction by affinity chromatography |
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Authors: | Yukinobu Hino Shigeki Minakami |
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Institution: | Department of Biochemistry, Kyushu University School of Medicine, Fukuoka 812, Japan |
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Abstract: | Hexose-6-phosphate dehydrogenase of rat liver microsomes was purified to an apparently homogeneous state with a recovery of about 36% using 8-aminooctyl Sepharose, DEAE-cellulose and 2′,5′-ADP Sepharose columns. This enzyme was insensitive to SH-reagent p-chloromercuribenzoate and oxidized galactose 6-phosphate, glucose 6-phosphate and glucose, with either NADP or NAD as an electron acceptor. The minimum molecular weight of this enzyme was estimated to be 104,000 in SDS-polyacrylamide gel electrophoresis in the presence of 2-mercaptoethanol. |
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