Effect of 1,2-dimethoxyethane on potato phosphorylase for polysaccharide specificity |
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Authors: | Joyce F Miller Donald J Graves |
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Institution: | Department of Biochemistry and Biophysics Iowa State University, Ames, Iowa 50011 USA |
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Abstract: | Potato phosphorylase normally utilizes starch as a substrate but will not use glycogen effectively. In the presence of 20% 1,2-dimethoxyethane, this specificity is lost because of marked activation with glycogen and some inhibition with starch. The effect of dimethoxyethane on β-amylase was the same for both starch and glycogen. These was no contaminating enzyme that would make glycogen more “starch-like.” The 20-fold decrease in Km for glycogen, a change in pH profile, and elimination of inhibition by cyclodextrin suggest that dimethoxyethane causes a change in phosphorylase structure. |
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