Polyamines are needed for the differentiation of 3T3-L1 fibroblasts into adipose cells |
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Authors: | Delia R. Bethell Anthony E. Pegg |
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Affiliation: | Department of Physiology, The Milton S. Hershey Medical Center The Pennsylvania State University, Hershey, Pennsylvania 17033 USA |
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Abstract: | When non-proliferating 3T3-L1 fibroblasts were stimulated to differentiate into adipose cells, there was a dramatic increase in the intracellular level of the polyamine, spermidine. Addition of α-difluoromethylornithine, an inhibitor of polyamine biosynthesis, depleted the cellular polyamines and prevented triglyceride accumulation and differentiation. The inhibitory effect of α-difluoromethylorinithine was completely abolished by provision of spermidine or putrescine. This suggests that polyamines are needed in the processes of differentiation as well as their established requirement for cell growth. |
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Keywords: | To whom correspondence should be addressed. |
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