Photophosphorylation in cell envelope vesicles from |
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Authors: | Robert D Clark Russell E MacDonald |
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Institution: | Section of Biochemistry, Molecular and Cell Biology Cornell University, Ithaca, New York 14853 USA |
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Abstract: | We have prepared vesicles from cell envelope membranes of strains R1 and ET-15 which are able to synthesize ATP in response to illumination. This photophosphorylation is inhibited by dicyclohexylcarbodiimide (DCCD) and by phloretin. ATP synthesis in L vesicles from the R1 strain (which contain bacteriorhodopsin) is inhibited by the protonophore 1799 but not by valinomycin. In M vesicles from the R1 strain and in ET-15 vesicles (both contain halorhodopsin) photophosphorylation is inhibited by both 1799 and valinomycin. These data are consistent with the idea that light-driven ATP synthesis can be coupled to the electrochemical H+ gradient generated by bacteriorhodopsin or by halorhodopsin through the membrane potential component of protonmotive force. |
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