Mutant cell lines resistant to azetidine carboxylic acid: Quantitative and qualitative differences in pyrroline-5-carboxylate synthase activity |
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| Authors: | RJ Smith RF Lodato DL Valle A Kazakis |
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| Institution: | 1. Howard Hughes Medical Institute at Harvard Medical School, Joslin Research Laboratory, Boston, Massachusetts 02215, USA;2. The Department of Medicine, Brigham and Women''s Hospital, Boston, Massachusetts 02215, USA.;3. Howard Hughes Medical Institute at Johns Hopkins University School of Medicine, Baltimore Maryland, USA.;4. The Department of Pediatrics, The Johns Hopkins Hospital, Baltimore Maryland, USA. |
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| Abstract: | Mutant Chinese hamster lung fibroblasts were selected that are resistant to the proline analog L-azetidine-2-carboxylic acid. Resistance in the two mutant cell lines is associated with two distinct alterations in pyrroline-5-carboxylate synthase, the enzyme that catalyzes the proline biosynthetic step leading from glutamic acid to pyrroline-5-carboxylate. In one mutant cell line, pyrroline-5-carboxylate synthase specific activity is increased 30-fold over the level in control cells. In the other mutant line, pyrroline-5-carboxylate synthase activity is not increased, but the enzyme has become insensitive to inhibition by ornithine and proline. |
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| Keywords: | AZCA L-azetidine-2-carboxylic acid P5C pyrroline-5-carboxylate MEM Eagle's minimum essential medium FCS fetal bovine serum OAB o-aminobenzaldehyde |
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