<sup>13</sup>C NMR evidence for three slowly interconverting conformations of the dihydrofolate reductase-NADP<sup>+</sup>-folate complex期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

¹³C NMR evidence for three slowly interconverting conformations of the dihydrofolate reductase-NADP⁺-folate complex

Authors:	B. Birdsall A. Gronenborn G.M. Clore G.C.K. Roberts J. Feeney A.S.V. Burgen

Affiliation:	Division of Molecular Pharmacology, National Institute for Medical Research, Mill Hill, London NW7 1AA, U.K.

Abstract:	The 3-carboxamido-¹³C resonance of NADP⁺ in its complex with $Lactobacillus casei$ dihydrofolate reductase and folate has been studied as a function of pH. At low pH a single resonance is observed, while at high pH two resonances are observed, neither of which has the same chemical shift as that seen at low pH. The rates of interconversion between the three states of the complex represented by these resonances are < 19 s^?1 at 11°C.

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