Intracellular phosphate pools show compartmentalization of intracellular pH in turtle urinary bladder |
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Authors: | Jose A.L. Arruda Harry Lubansky Sandra Sabatini George Dytko C.Tyler Burt |
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Affiliation: | 1. Department of Medicine, Section of Nephrology University of Illinois Hospital Chicago, Illinois 60612 USA;2. Department of Physiology and Biochemistry University of Illinois Hospital Chicago, Illinois 60612 USA;3. Veterans Administration West Side Hospital Chicago, Illinois 60612 USA |
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Abstract: | The urinary bladder of the fresh water turtle is capable of acidification and Na transport, in vitro, and it has been extensively used as a model of distal nephron of the kidney. In the course of measuring intracellular pH of stripped turtle bladder mucosa with phosphorus nuclear magnetic resonance, we observed the consistent presence of two inorganic phosphorus resonances under aerobic conditions, indicating the existence of a pH gradient possibly between cytosol and mitochondrion. This pH gradient was collapsed by addition of N2 and could be restored by reintroduction of oxygen. These observations demonstrate the existence of a spontaneous pH gradient between cytosol and mitochondria of turtle bladder epithelial cells. |
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