Calcium stimualtion of protein kinase C in the absence of added phospholipids |
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Authors: | Vivian S. Zabrenetzky Eleanor Bruckwick Walter Lovenberg |
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Affiliation: | Section on Biochemical Pharmacology National Heart, Lung and Blood Institute NIH, Bldg. 10, Room 7N262, Bethesda, MD 20205 USA |
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Abstract: | The activity of protein kinase C as isolated and described by Inoue, M., Kishimoto, A., Takai, Y., and Nishizuka, Y. (1977) J. Biol. Chem. , 7610–7616, can be markedly stimulated by Ca2+ in the presence of 4 mM Mg2+. This Ca2+ dependency does not require the presence of phospholipids or exogenous calmodulin. The increase in activity in the presence of Ca2+ is blocked by fluphenazine in the presence of 30 mM 2-mercaptoethanol. These results suggest that a calmodulin-like moiety may be a subunit of prokinase C. |
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