Physicochemical analysis of reversible molybdate effects on different molecular forms of glucocorticoid receptor |
| |
Authors: | T.William Hutchens Francis S. Markland Edward F. Hawkins |
| |
Affiliation: | 1. Department of Biochemistry, University of Southern California Los Angeles, CA 90033 USA;2. Department of Physiology and Biophysics University of Southern California Los Angeles, CA 90033 USA;3. the USC Comprehensive Cancer Center University of Southern California Los Angeles, CA 90033 USA |
| |
Abstract: | Several distinct molecular forms of glucocorticoid receptor have been identified in a melanoma model system. We have used velocity sedimentation to monitor molybdate dependent alterations in receptor size and heterogeneity. In the absence of molybdate, native glucocorticoid receptor from dexamethasone-sensitive tumors sediments at 7–8 S and 12–13 S. Under identical conditions, receptor isolated from dexamethasone-resistant tumors sediments at 7–8 S only. However, when molybdate is introduced, either during homogenization or immediately prior to centrifugation, glucocorticoid receptors from both dexamethasone-sensitive and -resistant tumors sediment sharply at 9–10 S. These molybdate induced phenomena are reversible. The activated forms of glucocorticoid receptor isolated from both dexamethasone-sensitive and -resistant tumors by DEAE-cellulose chromatography have similar sedimentation coefficients (4–5 S) which are unaffected by molybdate. |
| |
Keywords: | Author to whom correspondence should be addressed at: USC Cancer Center Research Building I 1720 Zonal Avenue Los Angeles California 90033. |
本文献已被 ScienceDirect 等数据库收录! |