Involvement of AMP-activated protein kinase in fat depot-specific metabolic changes during starvation |
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| Authors: | Sponarova Jana Mustard Kirsty J Horakova Olga Flachs Pavel Rossmeisl Martin Brauner Petr Bardova Kristina Thomason-Hughes Michaela Braunerova Radka Janovska Petra Hardie D Grahame Kopecky Jan |
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| Affiliation: | Department of Adipose Tissue Biology, Institute of Physiology, Academy of Sciences of the Czech Republic, Videnska 1083, 14220 Prague, Czech Republic. |
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| Abstract: | The mechanisms controlling fat depot-specific metabolism are poorly understood. During starvation of mice, downregulation of lipogenic genes, suppression of fatty acid synthesis, and increases in lipid oxidation were all more pronounced in epididymal than in subcutaneous fat. In epididymal fat, relatively strong upregulation of uncoupling protein 2 and phosphoenolpyruvate carboxykinase genes was found. In mice maintained both at 20 and 30 degrees C, AMP-activated protein kinase was activated in epididymal but did not change in subcutaneous fat. Our results suggest that AMPK may have a role in the different response of various fat depots to starvation. |
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| Keywords: | ACC, acetyl-CoA carboxylase AMPK, AMP-activated protein kinase α1 (α2) AMPK, α1 and α2 isoforms of the catalytic subunit of AMPK FA, fatty acids FAS, fatty acid synthase NEFA, non-esterified fatty acids PEPCK, phosphoenolpyruvate carboxykinase pACC, the phosphorylated form of ACC pAMPK, phosphorylated form of AMPK SREBP-1, sterol regulatory element-binding protein-1 UCP, uncoupling protein WAT, white adipose tissue |
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