Mouse ES cells maintained in different pluripotency-promoting conditions differ in their neural differentiation propensity |
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Authors: | Haruka Hirose Hidemasa Kato Akie Kikuchi-Taura Toshihiro Soma Akihiko Taguchi |
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Affiliation: | (1) Department of Regenerative Medicine, National Cerebral and Cardiovascular Center, 5-7-1 Fujishiro-dai, Suita Osaka, Japan, 565-8565;(2) Division of Developmental Biology, Research Center for Genomic Medicine, Saitama Medical University, 1397-1 Yamane, Hidaka Saitama, Japan, 350-1241;(3) Division of Hematology, Department of Internal Medicine, Hyogo College of Medicine, 1-1 Mukogawachou, Nishinomiya Hyogo, Japan, 663-850;(4) Department of Regenerative Medicine Research, Institute of Biomedical Research and Innovation, 2-2 Minatojima Minamimachi Chuo-ku, Kobe Hyogo, Japan, 650-0047; |
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Abstract: | Prior to differentiation, embryonic stem (ES) cells in culture are maintained in a so-called “undifferentiated” state, allowing derivation of multiple downstream cell lineages when induced in a directed manner, which in turn grants these cells their “pluripotent” state. The current work is based on a simple observation that the initial culture condition for maintaining mouse ES cells in an “undifferentiated” state does impact on the differentiation propensity of these cells, in this case to a neuronal fate. We point out the importance in judging the “pluripotency” of a given stem cell culture, as this clearly demonstrated that the “undifferentiated” state of these cells is not necessarily a “pluripotent” state, even for a widely used mouse ES cell line. We partly attribute this difference in the initial value of ES cells to the naïve-to-primed status of pluripotency, which in turn may affect early events of the differentiation in vitro. |
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