| 野生茄子（Solanum torvum）抗黄萎病相关基因Sto Vel的克隆与分析 |
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| 引用本文： | 史仁玖 殷明 王忠 陈敏 黄权生 黄乐平 杨清. 野生茄子（Solanum torvum）抗黄萎病相关基因Sto Vel的克隆与分析[J]. 植物生理学通讯, 2006, 42(4): 638-642 |
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| 作者姓名： | 史仁玖 殷明 王忠 陈敏 黄权生 黄乐平 杨清 |
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| 作者单位： | [1]南京农业大学生命科学学院,南京210095 [2]新疆农业科学院核生物技术研究所,乌鲁木齐830000 |
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| 基金项目： | 新疆农业科学院核生物技术研究所自治区重点实验室基金. |
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| 摘 要： | 采用RT—PCR和RAcE技术从野生茄子中扩增克隆到一个抗黄萎病相关基因，命名为StoVel，其cDNA全长3400bp，含有3153bp的完整开放阅读框，编码1051个氨基酸，该基因编码的蛋白序列与刚果野茄、类番茄和番茄Vel编码的氨基酸序列同源性分别为82％、81％和80％，且有很高的功能区段保守性。将该cDNA全长序列提交Gen Bank，登陆号为DQ020574。半定量PCR表明该基因为组成型表达，在根中表达最多，叶中最少。
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| 关 键 词： | 野生茄子 StoVel基因 克隆 表达 |
| 收稿时间： | 2006-01-26 |
| 修稿时间： | 2006-05-25 |
Cloning and Analysis of Full-length cDNA of StoVel Gene from Solanum torvum |
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| SHI Ren-Jiu, YIN Yue, WANG Zhong, CHEN Min, HUANG Quan-Sheng, HUANG Le-Ping, YANG Qing. Cloning and Analysis of Full-length cDNA of StoVel Gene from Solanum torvum[J]. Plant Physiology Communications, 2006, 42(4): 638-642 |
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| Authors: | SHI Ren-Jiu YIN Yue WANG Zhong CHEN Min HUANG Quan-Sheng HUANG Le-Ping YANG Qing |
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| Abstract: | The full-length cDNA of a gene associated with verticillium wilt disease resistance, named StoVe1,was amplified and cloned from Japanese Solanum torvum by RT-PCR and RACE. The cDNA contained 3 400bp with an open reading frame (ORF) of 3 153 bp and encoded 1 053 amino acid residues. The deduced aminoacid sequence showed 82% homology to SaVe1 from Solanum aethiopicum, 81% homology to SlVe1 fromSolanum lycopersicoides and 80% homology to Ve1 from Lycopersicon peruvianum and also highly conserveddomains of function. This gene has been registered in GenBank with accession number DQ020574. Semi-quantitive RT-PCR analysis indicated that StoVe1 expression was different in roots, stems and leaves, and richerin roots, poor in leaves. |
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| Keywords: | Solanum torvum StoVe l gene cloning expression |
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