Isolation and characterization of a sialo-glycopeptide from buffalo colostrum |
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Authors: | Huligerepura S Aparna Paramahans V Salimath |
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Institution: | (1) Department of Biochemistry and Nutrition, Central Food Technological Research Institute, 570 013 Mysore, India |
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Abstract: | A sialoglycopeptide was isolated from buffalo colostrum in pure form by chromatography on Sephadex G-25 and QAE-Sephadex A-25. This was found to be homogeneous by cellulose acetate membrane electrophoresis and reverse phase HPLC. It consisted of fucose, galactose, mannose,N-acetyl glucosamine andN-acetyl neuraminic acid in the ratio 1 2 3 4 1, and aspartic acid, serine, threonine, proline and glutamic acid were the major amino acids. Glycine was identified as the N-terminal amino acid residue. The structure elucidation of the carbohydrate moiety was carried out by methylation analysis, mass spectrometry,1H-NMR spectroscopy and the probable structure was revealed to be that of a complex biantennary type. |
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Keywords: | buffalo colostrum glycopeptide |
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