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Polymorphisme et caractère glycoprotéique des lectines de Ricinus communis purifiées par chromatographie d'affinité
Authors:M. Lhermitte   G. Lamblin   P. Degand  P. Roussel
Abstract:Two lectin fractions (So20 w = 6,8 and 4,9 S) were purified from Ricinus communis seeds. The purification was carried out in four steps : ammonium sulfate fractionation, affinity chromatography on Sepharose 4 B, gel filtration on Sephadex G 150 and chromatography on CM cellulose. The purified lectins were glycoproteins whose chemical composition was determined. Amino terminal analysis of the two fractions revealed glycine and serine. Polyacrylamide gel electrophoresis of the higher molecular weight fraction allowed the separation of several components with different affinity for PAS staining.
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