A novel nested multiplex polymerase chain reaction (PCR) assay for differential detection of <Emphasis Type="Italic">Entamoeba histolytica</Emphasis>, <Emphasis Type="Italic">E. moshkovskii</Emphasis> and <Emphasis Type="Italic">E. dispar</Emphasis> DNA in stool samples |
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Authors: | Krishna Khairnar Subhash C Parija |
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Institution: | (1) Department of Microbiology, Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER), Puducherry, India |
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Abstract: | Background
E. histolytica, a pathogenic amoeba, is indistinguishable in its cyst and trophozoite stages from those of non-pathogenic E. moshkovskii and E. dispar by light microscopy. We have developed a nested multiplex PCR targeting a 16S-like rRNA gene for differential detection of
all the three morphologically similar forms of E. histolytica, E. moshkovskii and E. dispar simultaneously in stool samples. |
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