Amino acid incorporation by preparations from the developing rat brain |
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| Authors: | D H Adams and L Lim |
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| Institution: | Department of Biochemistry, Institute of Psychiatry (University of London, British Postgraduate Medical Federation), Maudsley Hospital, Denmark Hill, London, S.E.5 |
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| Abstract: | 1. A study has been made of the ability of cerebral microsome-cell sap systems, taken from rats at various ages, to incorporate (14)C]valine. 2. The systems appear to follow Michaelis-Menten kinetics when cell sap is considered as substrate, microsomes as enzyme and total counts/mg. of microsomal protein as a measure of reaction velocity. 3. Reproducible ;affinity constant' values are obtained, and the system from 4-day-old rats has a higher V(max.) and affinity constant than the system from adult rats. 4. It is suggested the the amino acid-incorporating ability of different systems may be compared by this means. |
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