减压病山羊纤维蛋白原肽链结构与空间构象的研究

本文报道了减压病山羊纤维蛋白原(FG)结构变化及减压病(DCS)气-血界面活性引起凝血反应的作用。雄性山羊15只,加压-减压发生Ⅰ或Ⅱ型DCS,采静脉血用冷乙醇提取血浆FG。经SDS-PG电泳和CM_(22)-色谱分离S-磺酸化FG,发现FG裂解肽段——带4和X、Y峰,(正常对照组无);经HPLC和组分分析发现FG含量和FG氨基酸残基数明显减少(P<0.05),表明FG参入凝血反应其肽链发生裂解。又经圆二色谱分析发现FG α-hilex％明显下降(P<0.01)。DCS山羊FG结构的改变,证实了气-血界面活性作用引起凝血反应。

Studies on the Structure And Conformation of Peptide Chain in Fibrinogen of Goat in Decompression Sickness

In this paper, blood coagulation resulting from the structural changes in fibrinogen (FG) and gas-blood interface action of goat in decompression sickness was reported.15 male goats developed type **********I or *******II DCS after compression and decompression were studied.Plasma FG was extracted from blood by cold ethanol method.FG and S-sulfonyl FG were analyzed by SDS-PG electrophoresis and CM22 chroma- tography FC peptide fragments of band 4 and X,Y peaks were found.The FC con-tent and amino acid residues of FG were obviously decreased (P<0.05) as shown by HPLC and amino acid analysis.The results showed that degradation in the primary structure of FG due to blood coagulation had occured.CD analysis indicated that the percentage of ********a-helices in FG was decreased (p<0.01).The structral changes of FG confirm that blood coagulation caused by gas-blood interface action is a pathologic symptom in DCS.