| Abstract: | Intraperitoneal administration of 3H]-leukotriene E4 in the rat resulted in the appearance of radiolabel in urine and feces. Separation of polar urinary metabolites and chromatographic comparison of synthetic metabolites indicated the in vivo formation of ω-oxidized metabolites of LTE4 with sequential β-oxidation. Futhermore, the metabolite identified as 16-carboxy-17,18,19,20-tetranor-14,15-dihydro-N-acetyl-LTE4 substantiates the biochemical patheway of β-oxidation in vivo involving the 2,4-dienoyl CoA reductase as an integral step. These results substantiate β-oxidation of sulfidopeptide leukotrienes in vivo and these metabolites account for some of the major urinary metabolites of this class of lipid mediator. |
