Microwave irradiation increases recovery of neuropeptides from brain tissues |
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Authors: | Elvar Theodorsson, Carina Stenfors,Aleksander A. Math |
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Affiliation: | a Karolinska Institute Department of Clinical Chemistry, Karolinska Hospital, S-104 01, Stockholm, Sweden b Department of Psychiatry St Göran's Hospital, S-104 01, Stockholm, Sweden |
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Abstract: | The effect of focused high energy microwave treatment (MW) on brain concentrations and molecular forms of substance P, neurokinin A, neuropeptide Y, neurotensin, galanin and calcitonin gene-related peptide was investigated. Groups of rats were treated as follows: 1) MW, storage for 60 min at 22°C, 2) Decapitation, storage for 60 min at 22°C, 3) Decapitation, storage for 60 min at 22°C, MW treatment, 4) MW, decapitation, storage for 2 min at 22°C and 5) Decapitation, storage for 2 min at 22°C. Peptide concentrations were in all instances highest in the MW sacrificed groups. MW increased the concentration of intact peptides by rapid inhibition of peptidase activity and increase in peptide solubility/extractability. |
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Keywords: | Microwave irradiation Tachykinins Neuropeptide Y Neurotensin Galanin Calcitonin gene-related peptide |
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