Fertilization-induced changes in concanavalin A binding to mouse eggs |
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Authors: | M De Felici G Siracusa |
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Institution: | Istituto di Istologia e Embriologia generale, Università di Roma, 00161 Roma, Italy |
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Abstract: | The binding of concanavalin A (ConA) to zona-free unfertilized and fertilized mouse eggs has been investigated using tritiated ConA. At low lectin concentrations (1–5 μg ml?1) the fertilized egg shows a higher affinity for 3H]ConA than does the unfertilized egg. In saturation conditions, however, unfertilized and fertilized eggs show the same binding capacity (1.55 × 108 ConA molecules/egg). The results indicate that ConA-binding sites change qualitatively following fertilization; possible connections between this change and other fertilization-induced changes in the egg surface are discussed. |
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Keywords: | To whom offprint requests should be addressed |
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