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Modulation of surface proteins by calcium in Chinese hamster lung cells
Authors:KC Gupta  S Chatterjee  MW Taylor
Institution:Department of Biological Sciences, Indiana University, Bloomington, IN 47401, USA
Abstract:Investigations on the role of calcium in regulation of cell morphology of Chinese hamster lung cells (V79) revealed that cells grown with additional calcium (5 mM) in the growth medium (Ham's F12) adhere more tightly to the substratum than those grown in F12 alone. Additional calcium in the medium did not cause any changes in the structural membrane proteins or glycoproteins. Radioiodination of the surface membrane proteins of cells grown with or without additional calcium showed distinct differences in the labeling profile. The most striking change observed in cells grown with additional calcium was a very heavily labeled protein band at 70 K molecular weight. Two bands at approx. 100 K and 42 K were also heavily labeled. In contrast, the amount of radioactivity of a protein band at 52 K decreased in the cells grown in additional calcium. In general, cells grown with additional CaCl2 were better iodinated than those grown in growth medium alone. The results demonstrate that calcium modulates surface proteins of V79 cells and this modulation may account for the changes observed in the cell morphology.
Keywords:Present address: Division of Virology  St Jude Children's Research Hospital  Memphis  TN 38101  USA  to whom offprint requests should be addressed  
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