Scanning electron microscopy of the G-banded human karyotype |
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Authors: | Christine J. Harrison Martin Britch Terence D. Allen Rodney Harris |
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Affiliation: | 1. Department of Medical Genetics, St Mary''s Hospital, Manchester M13 OJH, UK;2. Department of Ultrastructure, Paterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester M20 9BX, UK |
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Abstract: | The chromosome structure of human metaphases was observed in the scanning electron microscope (SEM) after exposure to G-banding techniques for light microscopy (LM). Individual chromosomes showed an inherent specificity of quaternary coiling. Circumferential grooves along the chromatids demarcated the individual gyres of the coils, which were shown to correspond to the LM G-banding pattern. An increased number of quaternary coils was observed in prometaphase chromosomes, which were shown to be correlated with the high resolution LM bands. We propose that the observation of G-bands relies on LM visualization of quaternary structure by accumulation of Giemsa stain between the coils. |
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