The effects of extracellular sodium on acid release and motility initiation in rat caudal epididymal spermatozoa in vitro

When rat caudal epididymal spermatozoa were incubated in a sodium-free solution, they suffered a progressive fall in motility, and by 40 min the motility was completely suppressed. However, upon resuspending the spermatozoa in a sodium containing solution, motility was completely restored within 15 min. During this reinitiation period, H⁺ ions were found to be released from spermatozoa. Both motility reinitiation and acid release were found to be closely dependent on the extracellular sodium. Of the other monovalent cations studied, only NH₄⁺ could replace Na⁺ in these events. Both processes were partially inhibited by amiloride (10^?4-10^?3 M) and ouabain (10^?4-10^?3 M) but was unaffected by acetazolamide (10^?4 M). The motility activation and acid release were studied under various conditions. It was found that there was a close correlation between the two processes. The H⁺ efflux during motility activation was accompanied by a rise in the intracellular pH of the sperm. It is proposed that the requirement of Na⁺ for the motility initiation is attributed to an increase in the intracellular pH via the Na⁺-H⁺ exchange. An intracellular pH shift might be involved in motility activation in mammalian sperm.