Cell surface changes associated with EGF and insulin-induced cell adhesion |
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| Authors: | Judith A Berliner Aaron Aharanov Rebecca Pruss |
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| Institution: | 1. Laboratory of Nuclear Medicine and Radiation Biology, University of California, School of Medicine, Los Angeles, CA 90024, USA;2. Department of Pathology, University of California, School of Medicine, Los Angeles, CA 90024, USA;3. Department of Biological Chemistry, University of California, School of Medicine, Los Angeles, CA 90024, USA |
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| Abstract: | The ability of insulin and epidermal growth factor (EGF) to restore cell surface function in cells damaged by serum deprivation has been examined. Both insulin (10?7 M) and EGF (10?8 M), when added for 2 h, resulted in reattachment of cells to the culture dish; with insulin, attachment was associated with increased amino acid uptake. Both hormones caused an increase in sialic acid and in free sulfhydryl groups associated with the cell membrane. The mobility of concanavalin A (Con A) on the upper cell surface was increased by EGF, and to a lesser extent by insulin. The effects of insulin and EGF on adhesion appear to be regulated by specific receptors for these hormones. |
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