The accumulation of lens-specific protein and mRNA in cultures of neural retina from 312-day chick embryos |
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| Authors: | I. Thomson K. Yasuda D.I. De Pomerai R.M. Clayton T. Okada |
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| Affiliation: | 1. Department of Biological Sciences, Napier College, Edinburgh EH10 5DT, Scotland;2. Department of Biophysics, Faculty of Science, University of Kyoto, Kyoto 606, Japan;3. Department of Zoology, University of Nottingham, University Park, Nottingham, UK;4. Institute of Animal Genetics, University of Edinburgh, Edinburgh EH9 3JN, Scotland |
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| Abstract: | A detergent insoluble 58 000 D polypeptide is phosphorylated at an increased rate during mitotic arrest in cultured Chinese hamster ovary cells. Using one-dimensional peptide mapping, this polypeptide was identified as a phosphorylated form of intermediate filament protein (IFP). The increased rate of phosphorylation is observed with either colcemid- or vinblastine-treated mitotic cells, but is not seen with similarly treated interphase cells. |
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