A comparative evaluation of aflatoxin B1 genotoxicity in fish models using the Comet assay |
| |
Authors: | Gamal A. Abd-Allah Refaat I. El-Fayoumi Micah J. Smith Richard A. Heckmann Kim L. O'Neill |
| |
Affiliation: | a Department of Zoology, Damietta Fac. Sci., Mansoura University, Damietta, Egypt;b Department of Zoology, Fac. Sci., Mansoura University, Mansoura, Egypt;c 751 WIDB, Department of Microbiology, BYU, Provo, UT 84602, USA;d Department of Zoology, Brigham Young University, Provo, UT 84602, USA |
| |
Abstract: | Aflatoxin B1 (AFB1) is classified as a Group I hepatocarcinogen in humans by the International Agency for Research on Cancer (IARC). The alkaline Comet assay is a simple and rapid method by which DNA damage can be demonstrated as a function of tail moment. The present work is the first to evaluate the genotoxicity of AFB1 in fish using the Comet assay. Two different species of fish were selected as models due to previously established sensitivity to AFB1: rainbow trout (sensitive) and channel catfish (resistant). Fish were i.p. injected with 0.5 mg AFB1/1 ml DMSO/1 kg body weight. The Comet assay was performed after 4 and 24 h on whole blood, liver, and kidney cells of both species. Trout blood and kidney tissue tested displayed significant (p<0.05) and extensive DNA damage (shown by increased tail moment) after 4 h which then decreased by 24 h. In liver cells, damage progressively increased over time. Conversely, similarly treated catfish showed no elevation in DNA damage over controls at the same doses. These results suggest that the Comet assay is a useful tool for monitoring the genotoxicity of mycotoxins such as AFB1 and for evaluating organ specific effects of these agents in different species. |
| |
Keywords: | Aflatoxin B1 Alkaline Comet assay Hepatocarcinogen Fish |
本文献已被 ScienceDirect 等数据库收录! |
|