Diversity and exploration of bioactive marine actinomycetes in the Bay of Bengal of the Puducherry coast of India |
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Authors: | Krish Suthindhiran Krishnan Kannabiran |
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Institution: | (1) Department of Aquatic Biology and Fisheries, University of Kerala, Kariavattom campus, Trivandrum, 695 581, India;(2) 9/36, Second Street, Park Avenue, Thudiyalur, Coimbatore, 641 034, India |
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Abstract: | The present study was designed to investigate the Puducherry coast of the Bay of Bengal, India for the diversity of bioactive
actinomycetes. A total of 50 actinomycete strains were isolated from the marine sediments and most of the strains were belongs
to Streptomyces. These strains were identified by means of morphological physiological, biochemical and cultural characteristics. The isolates
were subjected to shake flask fermentation and the secondary metabolites were extracted with ethyl acetate and screened for
cytotoxicity, hemolytic activity and antimicrobial activity against selected bacterial and fungal pathogens. The cytotoxic
activity was evaluated using HeLa cell lines by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a tetrazole
(MTT) assay, hemolytic activity on mouse erythrocytes and the antifungal activity was evaluated by MTT cytotoxic assay against
Aspergillus niger, Aspergillus fumigatus and Candida albicans. The antibacterial activity was studied against Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Klebsiella pneumoniae. The cytotoxicity and antimicrobial activity of secondary metabolite was found to be concentration dependent and nearly 24%
of isolates showed significant antimicrobial, hemolytic and cytotoxic activity. The results of our study indicate the diversity
and bioactive potential of marine actinomycetes isolated in the Puducherry coast. |
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Keywords: | Actinomycetes Streptomyces Secondary metabolite Antagonism Cytotoxicity |
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