Adenylate cyclase activity in a higher plant, alfalfa (Medicago sativa). |
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| Authors: | V C Carricarte G M Bianchini J P Muschietti M T Téllez-I?ón A Perticari N Torres M M Flawiá |
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| Institution: | Instituto de Investigaciones en Ingeniería Genética y Biología Molecular (INGEBI-CONICET), Facultad de Ciencias Exactas y Naturales, UBA, Buenos Aires, Argentina. |
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| Abstract: | An adenylate cyclase activity in Medicago sativa L. (alfalfa) roots was partially characterized. The enzyme activity remains in the supernatant fluid after centrifugation at 105,000 g and shows in crude extracts an apparent Mr of about 84,000. The enzyme is active with Mg2+ and Ca2+ as bivalent cations, and is inhibited by EGTA and by chlorpromazine. Calmodulin from bovine brain or spinach leaves activates this adenylate cyclase. |
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