啤酒花节间外植体再生体系的建立

在国内首次建立了两个啤酒花主栽品种（麒麟丰禄，青岛大花）的节间外植体再生体系。实验表明：啤酒花无菌苗的节间为适于不定芽再生韵外植体；适宜的再生培养基为Ms基本培养基＋B5维生素＋葡萄糖20g／L，附加IAA0．25mg／L＋TDZ2mg／L（麒麟丰禄）或IAA0．125mg／L＋TDZ1．5mg／L（青岛大花），以7g／L琼脂固化，pH5．8；不定芽再生频率可达57％（麒麟丰禄）或16％（青岛大花）。啤酒花再生体系的建立，为利用植物基因工程技术培育抗病虫害啤酒花新品系奠定了基础。

Establishment of Regeneration Systems from Internode Explants of Hops(Humulus lupulus L.)

The regeneration systems from internode explants of two main cuhivars of hops （ Humulus lupulus L. ） are established for the first time in China. The experiments showed that： the shoot internodes from in vitro cultures of hops were the suitable explant type for shoot regeneration; the optimal regeneration medium was MS basal medium combined with B5 vitamins and glucose 20 g/L, supplemented with IAA 0.25 mg/L and TDZ 2 mg/L （ for Qilinfenglu） , or IAA 0. 125 mg/L and TDZ 1.5 mg/L （for Qingdaodahua） , and solidated with agar 7 g/L, pHS. 8; and the shoot regeneration frequency reached 57 % for Qilinfenglu and 16 % for Qingdaodahua in the separate optimal medium. The establishment of hop regeneration systems lays the foundation for the development of new hop lines with enhanced disease- and pest-resistance through plant genetic engineering techniques.