Preparation,characterization and laboratory-scale application of an immobilized glucoamylase |
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Authors: | B. Szajáni G. Klamár L. Ludvig |
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Affiliation: | 1. Reanal Factory of Laboratory Chemicals, Telepes utca 53, 1147, Budapest XIV, Hungary;2. Research Institute of Alcohol Industry, Diószeghy Sámuel utca 8, 1089 Budapest VIII, Hungary |
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Abstract: | Glucoamylase (1,4-α-d-glucan glucohydrolase, EC 3.2.1.3) has been covalently immobilized on a polyacrylamide-type support containing carboxylic groups activated by water-soluble carbodiimide. The activity was 5.5– 6.0 units g?1solid. The optimum pH for catalytic activity was pH 3.8. The apparent optimum temperature was found at 60°C. With soluble starch as substrate the Km value was 14 mg ml?1. The pH for maximum stability was pH 4.0–4.5. In the presence of 8 m urea the immobilized glucoamylase retained most of its catalytic activity but it was more susceptible to guanidinium hydrochloride than the soluble enzyme. The practical applicability of immobilized glucoamylase was tested in batch process and continuous operation. |
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Keywords: | Starch saccharification immobilized glucoamylase covalent attachment catalytic properties stability tests |
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