Dithiothreitol reactivates desacetoxycephalosporin C synthetase after inactivation |
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| Authors: | Claus Lübbe Saul Wolfe Arnold L. Demain |
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| Affiliation: | 1. Fermentation Research Laboratory, Massachusetts Institute of Technology, Cambridge, MA 02139, USA;2. Department of Chemistry, Queens University, Kingston, Ontario, Canada K7L 3N6 |
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| Abstract: | Dithiothreitol (DTT) has been found to stimulate the desacetoxycephalosporin C synthetase (expandase) activity of a frozen crude extract of Streptomyces clavuligerus, even in the presence of an optimum concentration of ascorbate. Catalase, both native and heat-denatured, and bovine serum albumin (BSA) also stimulated the enzyme activity but were less active than DTT. Although fresh extracts were sporadically stimulated, frozen extracts or extracts inactivated by shaking at 37°C in air were consistently activated. It is felt that DTT functions as a reactivating, rather than an activaing, agent. Similar effects were observed with extracts of Cephalosporium acremonium. |
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| Keywords: | Antibiotic biosynthesis cephalosporin C inactivation dithiothreitol desacetoxycephalosporin C synthetase |
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