首页 | 本学科首页   官方微博 | 高级检索  
   检索      


Kinetic studies of the enzymatic isomerization of xylose
Authors:Graciela N Roman  Norman B Jansen  Humg-Yu Hsiao  George T Tsao
Institution:Laboratory of Renewable Resources Engineering, Purdue University, West Lafayette, IN 47907, USA
Abstract:d-Xylose isomerase catalyses the conversion of the common pentose, d-xylose, to its keto-isomer, d-xylose. This reaction is of interest because many microorganisms that are unable to metabolize d-xylose can utilize d-xylulose. The kinetics of a commonly used immobilized whole-cell isomerase, Sweetzyme Q, have been determined from initial rate studies on the forward and reverse reactions. The effect of pH, temperature, and substrate and product concentration on enzyme activity have all been examined. Reaction rates were modelled with the Michaelis-Menten equation. Using constants determined from Lineweaver-Burk plots, the rate equation accurately simulated experimental conversion data.
Keywords:Kinetics  xylose isomerase  xylulose
本文献已被 ScienceDirect 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号