Alkali process for enhancing susceptibility of autohydrolysed beech sawdust to enzymatic hydrolysis |
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Authors: | Zdzisław Targoński |
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Affiliation: | Department of Food Technology, Agricultural University, 20-934 Lublin, Akademicka 13, Poland |
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Abstract: | Autohydrolysed beech sawdust has been treated with aqueous NaOH solution in a three-stage process to increase the susceptibility of cellulose to cellulolytic enzymes. This process consisted of neutralization of autohydrolysed wood, extraction of lignin and alkali treatment of residual solids with 1.5% aqueous NaOH solution at 135°C for 1 h. The cellulose in the residues was then hydrolysed with Novo (SP 122) and Fusarium sp. 27 cellulases [see 1,4-(1,3;1,4)-β-d-glucan 4-glucanohydrolase, EC 3.2.1.4]. The susceptibility of cellulose to cellulases was increased 2.3 to 2.7-fold. |
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Keywords: | Cellulose cellulase beech sawdust autohydrolysis, alkali treatment |
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