Urinary Peptidomic Analysis Identifies Potential Biomarkers for Acute Rejection of Renal Transplantation |
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Authors: | Tara K Sigdel Xuefeng B Ling Ken H Lau Li Li James Schilling Minnie M Sarwal |
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Institution: | (1) Division of Nephrology, Department of Pediatrics, Stanford University Medical School, Stanford University, Stanford, CA 94305, USA;(2) Divisions of Immunology, Department of Pediatrics, Stanford University Medical School, Stanford University, Stanford, CA 94305, USA;(3) Stanford University Medical School, G306, 300 Pasteur Drive, Stanford, CA 94304, USA |
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Abstract: | Introduction Human urine is a complex matrix of proteins, endogenous peptides, lipids, and metabolites. The level of any or all of these
components can reflect the pathophysiological status of an individual especially of the kidney at the time of urine collection.
The naturally occurring endogenous urinary peptides which are thought to be the product of several proteolytic and degradation
processes may provide clinically useful biomarkers for different renal and systemic diseases.
Materials and Methods To examine if specific differences in the urinary peptidome (<10 kDa) occur at the time of acute renal transplant rejection
(AR), we undertook a study of urine samples collected from biopsy-proven AR (n = 10), stable graft function (n = 10), and healthy normal control (n = 10). The peptides (<10 kDa) were extracted and fractionated with high-performance liquid chromatography followed by matrix-assisted
laser desorption/ionization (MALDI) time-of-flight mass spectrometric (MS) analysis.
Results We identified 54 endogenous peptides, including multiple peptides for Tamm–Horsfall protein (UMOD). A panel of peptides are
identified which discriminate renal transplant patients with AR from stable graft. We have shown that liquid chromatography
followed by MALDI is a useful tool to identify potential biomarkers, which after verification with larger patient cohort can
be used as a non-invasive monitoring tool for renal transplant rejection. |
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Keywords: | Urine Peptidomics Biomarker Renal transplantation Proteomics LC MALDI Urinary Peptidomics Acute rejection Non-invasive biomarkers Biomarker discovery |
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