Isolation and characterization of a new fimbrial antigen (CS1541) from a porcine enteroxigenic Escherichia coli O8: KX105 strain |
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Authors: | André Broes John M Fairbrother Mario Jacques Serge Larivière |
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Institution: | Département de Pathologie et Microbiologie, Facultéde Médecine Vétérinaire, Universitéde Montréal, Saint-Hyacinthe, Québec, Canada |
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Abstract: | Abstract A fimbrial antigen (CS1541) was extracted and purified from the porcine enterotoxigenic Escherichia coli strain 1541P (O8:K-:H9). CS1541 fimbriae appeared as long thin filaments 3–5 mm in diameter. CS1541 antigen consisted of two peptide bands of about 18 and 19 kDa as demonstrated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting. It was expressed both at 37°C and 15°C and did not demonstrate hemagglutinating properties. It was antigenically distinct from the fimbrial antigens K88, K99, F41, FY(Att25), F165, type 1, CFA-I, and CFA-II complex but demonstrated serological cross-reactions with the 987P fimbrial antigen. |
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Keywords: | Fimbria Adhesin Diarrhea Escherichia coli |
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