Adenosine triphosphate compartmentation in the rat heart: a 31P spin-lattice relaxation study |
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| Authors: | E Suzuki M Maeda S Kuki M C Steward H Takami Y Seo M Murakami H Watari |
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| Institution: | Department of Molecular Physiology, National Institute for Physiological Sciences, Aichi. |
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| Abstract: | Longitudinal relaxation times (T1) of phosphorus compounds in the perfused rat heart and erythrocytes were measured using the 31P Driven-Equilibrium Single-Pulse Observation of T1 relaxation (DESPOT) method at 33 degrees C. Both creatine phosphate in the heart and the three phosphate groups of adenosine triphosphate (ATP) in erythrocytes showed single-exponential relaxation. The three phosphate groups of ATP in the heart, however, had two T1 components. The T1 values of the short and the long T1 components of the beta-phosphate of ATP were ca. 0.4 and 14 s, respectively. The fraction with the long T1 represented ca. 30% of the total ATP content. These results suggested that there were two major pools of intracellular ATP in the rat heart which could be determined by 31P NMR spectroscopy. |
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