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A micro method for simultaneous determination of galactosyltransferase and 5''-nucleotidase activities in cell fractions.
Authors:L S Freilich   M E Richmond   A C Reppucci   Jr     J E Silbert
Abstract:Galactosyltransferase and 5'-nucleotidase were assayed in the same reaction mixture, with ovalbumin as exogenous acceptor of (14-C)galactose and with (3-H)AMP as the substrate for the 5'-nucleotidase assay. The substrates and reaction products of either assay had no significant effect on the activity of the other enzyme.
Keywords:
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