| 猕猴桃实生苗再生体系的建立 |
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| 引用本文： | 周玲艳,秦华明,赖幸韵,陈楚欢,梁 红. 猕猴桃实生苗再生体系的建立[J]. 广西植物, 2009, 29(4): 514-517 |
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| 作者姓名： | 周玲艳 秦华明 赖幸韵 陈楚欢 梁 红 |
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| 作者单位： | 仲恺农业工程学院,生命科学学院,广州,510225;华南农业大学生命科学学院,广州,510624;暨南大学理工学院,广州,510623;仲恺农业工程学院,生命科学学院,广州,510225 |
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| 基金项目： | 广东省科技计划项目，仲恺农业工程学院校基金 |
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| 摘 要： | 以成熟饱满的美味猕猴桃种子发芽获得实生苗,分别以实生苗的茎段、叶柄和叶片为材料建立了再生体系。结果表明:种子以2.5mg/L的赤霉素(GA3)处理8h较适合;以培养基发芽较为适合;茎段、叶柄和叶片的愈伤组织的诱导率均为100%,且茎段、叶柄比叶片容易脱分化,但叶片的平均出苗率最高。再生苗在移栽5d后,开始长出新叶,10d后就能完全适应外界环境。
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| 关 键 词： | 猕猴桃 实生苗 外植体 再生体系 |
Establishment of regeneration system of kiwifruit seedlings |
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| ZHOU Ling-Yan,QIN Hua-Ming,LAI Xing-Yun,CHEN Chu-Huan,LIANG Hong. Establishment of regeneration system of kiwifruit seedlings[J]. Guihaia, 2009, 29(4): 514-517 |
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| Authors: | ZHOU Ling-Yan QIN Hua-Ming LAI Xing-Yun CHEN Chu-Huan LIANG Hong |
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| Affiliation: | 1.College of Life Science, Zhongkai Agriculture and Engiineering University, Guangzhou 510225, China; 2.College of Life Science, South China Agricultural University, Guangzhou 510642, China; 3.College of Science and Technology, Jinan University, Guangzhou, 510632, China |
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| Abstract: | The full and mature seeds of kiwifruit were germinated to obtain the seedlings,and the regeneration system was established by using the stems,petioles and leaves as explants. The results showed that:the concentration of GA3 which was appropriate to treat the seeds was 2.5 mg/L and the treatment time was 8 h;the medium was more suitable for seed germination;the stems and petioles was easier to dedifferentiation than leaves,however,the average emergence rate of leaves was the highest. |
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| Keywords: | kiwifruit seedling explants regeneration system |
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