TUF factor binds to the upstream region of the pyruvate decarboxylase structural gene (PDC1) of Saccharomyces cerevisiae |
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| Authors: | Geraldine Butler Ian W Dawes and David J McConnell |
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| Institution: | (1) Department of Genetics, Lincoln Place Gate, Trinity College, Dublin 2, Ireland;(2) Department of Microbiology, University of Edinburgh, West Mains Road, EH9 MG Edinburgh, UK;(3) Present address: Department of Biological Chemistry, University of Michigan Medical School, 48109-0606 Ann Arbor, Michigan, USA;(4) Present address: Department of Biochemistry, University of New South Wales, P.O. Box 1, 2033 Kensington, New South Wales, Australia |
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| Abstract: | Summary The upstream activation site of the pyruvate decarboxylase gene, PDC1, of Saccharomyces cerevisiae contains an RPG box, and mediates the increase in expression of a PDC1-lacZ fusion gene during growth on glucose. Oligonucleotide replacement experiments indicate that the RPG box functions as an absolute activator of expression, but other elements (possibly CTTCC repeats) are required for carbon source regulation, and maximal expression. Gel retardation and oligonucleotide competition experiments suggest that the DNA binding factor TUF interacts with the RPG box in the upstream region of PDC1. Binding of TUF factor is not carbon source dependent in in vitro experiments, and is probably not responsible for glucose induction of PDC1 expression. |
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| Keywords: | Saccharomyces cerevisiae Gene regulation TUF Pyruvate decarboxylase |
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