Rotating simplex method of optimization of physical parameters for higher production of extracellular pectinases in bioreactor |
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Authors: | T. Panda G. S. N. Naidu |
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Affiliation: | Biotechnology Research Centre, Indian Institute of Technology-Madras, Chennai – 600 036, India, IN
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Abstract: | Rotating simplex method of optimization has been successfully used to determine the optimal conditions for extracellular pectinase production by Aspergillus niger in batch bioreactor. Maximum production of 1.132 U of polymethylgalacturonase, 1.313 U of polygalacturonase and 0.0091 U of pectinlyase was obtained at pH (controlled). 3.38; agitation rate, 483 rpm and aeration rate, 2.12 vvm. These conditions were found to be more suitable for pectinase production. |
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