Intracellular [Ca2+] and K+ and Cl- efflux responses in submandibular cells of neonatal and adult rats. |
| |
Authors: | J C Seagrave R C Ramsey S Barker J R Martinez |
| |
Institution: | Lovelace Medical Foundation, Center for Basic and Applied Medical Research, Albuquerque, NM 87106. |
| |
Abstract: | The effects of graded doses (5 x 10(-8) to 10(-5)) acetylcholine on intracellular Ca2+ and on 86Rb and 36Cl efflux were compared in submandibular cell clusters of 1 and 7 day-old and adult rats. Initial Ca2+ peaks were similar at agonists concentrations lower than 10(-7) M but the release of Rb+ and Cl- were smaller in cells of young animals. At higher agonist concentrations, Ca2+ peaks were higher in immature cells; however, initial Cl- (but not Rb+) efflux was similar to that of mature cells. Plateau Ca2+ levels were independent of age and agonist concentrations but the content of Cl- and Rb+ varied greatly and differences between age groups were less evident. These data confirm a dissociation between intracellular Ca2+ levels and Ca(2+)-mediated ion transport in immature salivary cells. |
| |
Keywords: | |
|
|