提高沙田柚茎尖嫁接成活率的研究

研究了可以影响沙田柚茎尖嫁接成活率的部分因素。结果表明 :当沙田柚接穗选择带 4个叶原基的茎尖、枳壳砧木选择黑暗培养 1 4d的实生苗或培养基中蔗糖浓度选择 7.5 %时各自的嫁接成活率较高。若在茎尖嫁接切口外缠绕 parafilm胶可令成活率明显增加。若在培养基中加入 GA3、6-BA和 IBA则明显抑制嫁接成活率。GA3、6-BA和 IBA处理接穗和砧木均可提高嫁接成活率 ,其中以 1 0 mg/L GA3处理效果最好 ,嫁接成活率可达 45 % ;6-BA和 IBA处理虽然也可提高嫁接成活率 ,但是同时又增加了接穗的脱落死亡率。

Studies on improving the successful frequency ofshoot-tip grafting of Shatian Pomelo

Some factors can affect the successful frequencies of shoot-tip grafting of Shatian Pomelo were studied. It is suggested that the more successful frequency of grafting was respectively obtained by using the meristems plus 4 leaf primordia as scions, using 2-week-old dark grown seedlings as rootstocks or choosing liquid culture media with 7 5% sugar concentration. The successful frequency of grafting could be increased obviously if we twisted the grafting-incisions with parafilm. It was obviously restrained if we put GA 3, 6-BA or IBA into liquid culture media. Those treatments with GA 3, 6-BA and IBA on scions and rootstocks all increased the successful frequency of grafting. The effect of 10 mg/L GA 3 was the best, and its successful frequency of grafting reached 45%. 6-BA and IBA could also advanced the successful frequency of grafting, but increaseed the shedding rate inducing death at the same time.