Temperature-dependent dissociation of Lucké renal adenocarcinoma cells |
| |
| Authors: | Errol D Seppanen Robert G McKinnel David Tad Louise A Rollins-Smith William Hanson |
| |
| Institution: | Department of Genetics and Cell Biology, University of Minnesota, Saint Paul, MN 55108, USA;Nuffield Department of Pathology, John Radcliffe Hospital, University of Oxford, Oxford OX3 9DU, England;Department of Microbiology, University of Rochester Medical Center, Rochester, NY 14642, USA |
| |
| Abstract: | Abstract. Fragments of Lucké renal adenocarcinoma were subjected to dissociation by rapid shaking after exposure to a divalent cation-free electrolyte solution, with or without 5 × 10−4 ethylenediaminetetraacetate (EDTA), at 7° C and 28° C. More cells detached at 28° C than at 7° C. Dissociation of cells from normal mesonephros fragments was minimal at both temperatures. It has been shown elsewhere that this frog tumor elaborates collagenase in a temperature-dependent manner. More collagenase is detected at 30° C than at 7° C. Normal kidney elaborates low levels of collagenase at both temperatures. Because our results suggested the possibility that some dissociation of the tumor cells may have been attributable to tumor-elaborated collagenase, we studied the effect of two collagenase inhibitors on dissociation. Both EDTA at high concentration and cysteine inhibit collagenase and both diminished tumor-cell dissociation. |
| |
| Keywords: | |
|
|
