Abstract: | The incidence of hydrocarbon-oxidizing microorganisms in water was determined by the method of plating on solid media. Vertical distribution of hydrocarbon-oxidizing microflora was different near the shore and in the open ocean; the incidence of the microorganisms was low in the surface water layer but increased at depths of 25 and 75 m in the open ocean in contrast to regions near the shore. Pure bacterial and fungal cultures were isolated and their properties were described. The cultures were grown in a liquid mineral medium with diesel fuel and 50 cultures out of 66 were found to be true hydrocarbon-oxidizing microorganisms surviving under the laboratory conditions. It was shown that bacteria and fungi have grown on tarballs collected from the surface of the ocean during their incubation at 30 degrees C. |