In vitro formation of lacuna structure by human dermal fibroblasts co-cultured with porcine chondrocytes on a 3D biodegradable scaffold |
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Authors: | Xia Liu Guangdong Zhou Wei Liu Wenjie Zhang Lei Cui Yilin Cao |
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Affiliation: | (1) Department of Plastic and Reconstructive Surgery, Shanghai 9th People’s Hospital, Shanghai Jiao Tong University School of Medicine, 200011 Shanghai, China |
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Abstract: | Dermal fibroblasts (DF) possess chondrogenic differentiation potential but whether DF, or a subpopulation of DF, can form a typical cartilage structure in culture is unknown. In this study, human DF were co-cultured with porcine articular chondrocytes on a biodegradable scaffold of polylactic acid/polyglycolic acid. Histological analysis demonstrated that some DFs can be induced to form cartilage lacuna structure showing the existence of a chondrogenic subpopulation of human DF. Moreover, the 3D-co-culture system can serve as an optimal model for directing stem cell differentiation in vitro. Xia Liu and Guangdong Zhou contributed equally to this work. |
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Keywords: | Chondrocytes Chondrogenesis Co-culture Dermal fibroblasts Scaffold of polylactic acid on polyglycolic acid |
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