Effects of cyclic mechanical stimulation of the cellular components of the heart: In vitro |
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Authors: | Louis Terracio Bonnie Miller Thomas K Borg |
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Institution: | (1) Department of Anatomy, University of South Carolina, 29208 Columbia, South Carolina;(2) Department of Pathology, University of South Carolina, 29208 Columbia, South Carolina |
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Abstract: | Summary The response of the cellular components of the heart to cyclic mechanical stimulation is of particular importance because
these cells are continually subjected to mechanical forces as a result of changes in blood volume and pressure. To directly
investigate how mechanical tension affects these cellular components of the heart, an in vitro system that exposes the particular
cell type (cardiac myocytes, endothelial cells, or fibroblasts) to a calibrated increase in cyclical linear stretch was developed.
Cells were grown on silastic membranes coated with laminin and subjected to a 10% cyclical distention 10 times a minute for
72 h. Within 24 h of being exposed to the mechanical stretch, the cells became elongated and oriented perpendicular to the
direction of the stretch. These results indicate that cyclical mechanical stimulation directly influences the cellular organization
of the heart cells in vitro.
This work was supported in part by grants HL 33656 and HL 24935 from the National Institutes of Health, Bethesda, MD. |
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Keywords: | cardiac myocytes fibroblasts endothelial cells cytoskeleton mechanical stimulation |
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