Newly established cell lines fromDrosophila larval CNS express neural specific characteristics |
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Authors: | Kumiko Ui Shoko Nishihara M Sakuma S Togashi R Ueda Y Miyata T Miyake |
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Institution: | (1) Laboratory of Cell Biology, Mitsubishi Kasei Institute of Life Sciences, 11 Minamiooya, Machida-shi, 194 Tokyo, Japan;(2) Present address: Department of Pharmacology, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, 113 Tokyo, Japan;(3) Present address: Laboratory of Cell Biology, Institute of Life Science, Soka University, 1-236 Tangi-cho, Hachioji-shi, 192 Tokyo, Japan |
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Abstract: | Summary From the central nervous system ofDrosophila melanogaster 3rd instar larvae, eight continuous cell lines have been established (named ML-DmBG1 to 8). Using ML-DmBG2, single colony
isolation was carried out and six colonial clones were obtained. All reacted to the antibody to horseradish peroxidase, which
is a neuronal marker in insects. Acetylcholine, a known neurotransmitter inDrosophila, was detected in three of the colonial clones by high performance liquid chromatography. Therefore, it is concluded that
the established colonial clones are neural cells originating in the larval central nervous system. Among them, some variation
was observed with respect to morphology, acetylcholine content, and reactivity to anti-HRP. The variation may reflect the
heterogeneity of cells composing the central nervous system. |
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Keywords: | Drosophila central nervous system cell lines anti-HRP antibody acetylcholine |
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